Layers/Functional-annotations.md

@@ -6,6 +6,7 @@ title: Functional annotations
 
 
 
+
 Functional annotation helps bringing biological meaning to genetic sequences. Functional annotation is usually obtained through protein sequence similarity. Indeed, across two organisms, if two sequences are very similar, one can infer that they can encode for the same biological function.
 There are several main parameters that will impact the process of functional annotation:
 - how distant is the species which was actually annotated with experimental data (the reference)
@@ -57,4 +58,11 @@ The **online** annotation tool TRAPID (https://bioinformatics.psb.ugent.be/trapi
 3. Name it and select `PLAZA 4.5 dicots` (or above) as the `Reference database`
 4. Click on the new experiment name
 5. Click on `Import data` on the left-side panel
-6. 
+6. Add your transcript file (can be .gzip)
+7. Click on `ADD FILE / URL`
+8. Click on `LOAD DATA INTO DATABASE`
+9. Wait for the Status to switch from `loading_db` to `upload`
+10. Check the number of processed transcripts
+11. Click on `PROCESS TRANSCRIPTS`
+![process_transcripts](uploads/9025b099e10cacfacfc62f3c8ae56230/image.png)
+14. Wait for the Status to switch from `processing` to `finished`